GSDME Knockout cell line(VERO C1008 (E6))

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location： Home > Products > KO Cell Lines > GSDME Knockout cell line(VERO C1008 (E6))

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GSDME Knockout cell line(VERO C1008 (E6)) Order

GSDME Knockout cell line(VERO C1008 (E6))
Catalog#
YKO-R005
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSDME Knockout cell line(VERO C1008 (E6))
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSDME Knockout cell line (VERO C1008 (E6))
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Green monkey
Gene Name
GSDME
Gene ID
103226364
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSDME gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the VERO C1008 (E6) cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSDME gene knockout GSDME Knockout cell line (VERO C1008 (E6)) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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